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Napierala and colleagues describe the utility of a friedreich’s ataxia cell line model, expressing an extremely low level of frataxin, for in vitro potency assays Unlike biochemical assays, these assays preserve the intricate interactions between cellular components, as well as native substrates at physiological. Cellular phenotypes are rescued upon exogenous frataxin expression, demonstrating the suitability of this model for testing gene therapy and protein supplementation approaches for friedreich’s ataxia.
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Cell migration and invasion assays cell migration is the movement of cells in response to specific external signals, including chemical and mechanical stimuli However, cell based assays can be grouped into categories such as cell proliferation assays, cell death assays, reporter gene assays, and cell signaling assays. During cell invasion, cells can modulate the environment as they migrate through the structure of the extracellular matrix (ecm)
Cell based assays are defined as any assay that takes place within a living cell
Because this definition is so general, there are thousands of different cell based assays
